Hotspot

rpl33-rps18

LSC · 69201-69367 · 167 bp

Back to Agapanthus

Pi 0.00808
MarkerSeek score 58.70
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
167
variable_sites
2
pi
0.00808
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TGTCGCTATTGTCACAAGCA TCGATCCCCCGATTCAATTG 45.0 50.0 57.8 57.7 294-296 bp 1.0 77.8
rpl33-rps18_p2 TGTCGCTATTGTCACAAGCA CAATTCGATCCCCCGATTCA 45.0 50.0 57.8 57.7 298-300 bp 1.0 77.7
rpl33-rps18_p3 TGTCGCTATTGTCACAAGCA TCAATTCGATCCCCCGATTC 45.0 50.0 57.8 57.5 299-301 bp 1.0 75.4
rpl33-rps18_p4 CGACACAATACACCCAGTCG CAATTCGATCCCCCGATTCA 55.0 50.0 58.7 57.7 337-339 bp 1.0 73.7
rpl33-rps18_p5 CGACACAATACACCCAGTCG TCGATCCCCCGATTCAATTG 55.0 50.0 58.7 57.7 333-335 bp 1.0 73.7