Hotspot

psaA-ycf3

LSC · 42599-43196 · 598 bp

Back to Agapanthus

Pi 0.00223

MarkerSeek score 60.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 2
pi: 0.00223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	704 bp	1.0	78.2
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	CTTGGTCCGATCAAACTGCT	50.0	50.0	57.9	58.2	784 bp	1.0	78.0
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GGTCCGATCAAACTGCTGAA	50.0	50.0	57.9	58.2	781 bp	1.0	77.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	708 bp	1.0	77.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	703 bp	1.0	77.1