Hotspot

trnT-GGU-psbD

LSC · 32623-33710 · 1088 bp

Back to Agapanthus

Pi 0.00123

MarkerSeek score 59.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 2
pi: 0.00123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGGACCAGCCTACAAAAAT	55.0	50.0	58.1	58.2	1249-1250 bp	1.0	78.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGGACCAGCCTACAAAAAT	50.0	50.0	58.1	58.2	1252-1253 bp	1.0	78.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1221-1222 bp	1.0	77.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1224-1225 bp	1.0	77.5
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	CCGGACCAGCCTACAAAAAT	50.0	50.0	58.3	58.2	1226-1227 bp	1.0	76.6