Hotspot

trnC-GCA-petN

LSC · 28564-29252 · 689 bp

Back to Agapanthus

Pi 0.000969
MarkerSeek score 59.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
689
variable_sites
1
pi
0.000969
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTA TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.5 58.0 770 bp 1.0 76.0
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTA TTAGTAGTGCCTTCAGCCCT 50.0 50.0 57.5 58.0 844 bp 1.0 75.6
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTA AGTCCACTCCTTCCCCATAC 50.0 55.0 57.5 58.1 822 bp 1.0 75.0
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTA GTCCACTCCTTCCCCATACT 50.0 55.0 57.5 58.1 821 bp 1.0 75.0
trnC-GCA-petN_p5 TTTTTGATCGGATTTGGCGG TTAAAGCAGCCCAAGCAAGA 45.0 45.0 57.4 58.0 809 bp 1.0 75.0