Hotspot

rpoC2

LSC · 17809-21849 · 4041 bp

Back to Afzelia

Pi 0.00385

MarkerSeek score 42.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4041
variable_sites: 37
pi: 0.00385
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCTCCCGTTTTCGTCCATT	CGCGTCTACTCAACAATGGA	45.0	50.0	57.4	58.0	240-243 bp	1.0	85.4
rpoC2_p2	AATTCTCCCGTTTTCGTCCA	CGCGTCTACTCAACAATGGA	45.0	50.0	57.4	58.0	242-245 bp	1.0	85.3
rpoC2_p3	TGTTACTGCATAGATCGGGC	CGCGTCTACTCAACAATGGA	50.0	50.0	57.5	58.0	267-270 bp	1.0	84.6
rpoC2_p4	ATTCTCCCGTTTTCGTCCAT	CGCGTCTACTCAACAATGGA	45.0	50.0	57.2	58.0	241-244 bp	1.0	83.5
rpoC2_p5	TGTTACTGCATAGATCGGGC	ACGCGTCTACTCAACAATGG	50.0	50.0	57.5	58.3	268-271 bp	1.0	82.3