Hotspot

ndhA

SSC · 124982-127288 · 2307 bp

Back to Afzelia

Pi 0.00477

MarkerSeek score 61.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2307
variable_sites: 23
pi: 0.00477
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	57.6	56.5	2414-2456 bp	1.0	41.4
ndhA_p2	TGAACTCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGAA	37.5	42.9	57.6	57.2	2414-2456 bp	1.0	41.4
ndhA_p3	ACCATAAGGAACATATCGTGA	TGGGAGAAGTTGATCGTTGA	38.1	45.0	54.2	56.5	2403-2445 bp	1.0	41.4
ndhA_p4	TCATGAACTCAGTTACCATAAGGA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	57.1	56.5	2417-2459 bp	1.0	41.4
ndhA_p5	TGAACTCAGTTACCATAAGGAACA	ATGGGAGAAGTTGATCGTTGA	37.5	42.9	57.6	57.0	2415-2457 bp	1.0	41.4