Hotspot

ccsA

SSC · 119370-120341 · 972 bp

Back to Afzelia

Pi 0.00185

MarkerSeek score 46.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 4
pi: 0.00185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	CTCGACGAAATGGGTGGAAT	50.0	50.0	58.0	58.0	1811-1820 bp	1.0	81.8
ccsA_p2	TGAAATCGGTAGACACGCTG	CTCGACGAAATGGGTGGAAT	50.0	50.0	58.0	58.0	1810-1819 bp	1.0	81.8
ccsA_p3	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.0	58.0	1751-1760 bp	1.0	81.7
ccsA_p4	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.0	58.0	1750-1759 bp	1.0	81.7
ccsA_p5	GTGAAATCGGTAGACACGCT	CGAAATGGGTGGAATGGCTA	50.0	50.0	58.0	57.9	1806-1815 bp	1.0	81.6