Hotspot

rpl32-trnL-UAG

SSC · 117828-119194 · 1367 bp

Back to Afzelia

Pi 0.0114
MarkerSeek score 63.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1367
variable_sites
31
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCACATCTCTTTCTACGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 766-1598 bp 1.0 85.3
rpl32-trnL-UAG_p2 GCCACATCTCTTTCTACGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 767-1599 bp 1.0 85.3
rpl32-trnL-UAG_p3 ACGGGGAACTCAAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 751-1583 bp 1.0 85.3
rpl32-trnL-UAG_p4 ACGGGGAACTCAAAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 752-1584 bp 1.0 85.2
rpl32-trnL-UAG_p5 GCCACATCTCTTTCTACGGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 776-1608 bp 1.0 84.4