Hotspot

rbcL-accD

LSC · 60413-61300 · 888 bp

Back to Afzelia

Pi 0.00783

MarkerSeek score 63.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 14
pi: 0.00783
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TTAGTTAGCCCACACCCGTA	50.0	50.0	58.0	58.1	865-1035 bp	1.0	84.3
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTAGTTAGCCCACACCCGTA	55.0	50.0	58.0	58.1	880-1050 bp	1.0	84.3
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	TAGTTAGCCCACACCCGTAT	50.0	50.0	58.0	57.8	864-1034 bp	1.0	83.5
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TAGTTAGCCCACACCCGTAT	55.0	50.0	58.0	57.8	879-1049 bp	1.0	83.5
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	GCCCACACCCGTATTCTAAC	50.0	55.0	58.0	58.3	858-1028 bp	1.0	82.1