Hotspot

psbZ-trnG-UCC

LSC · 39090-39834 · 745 bp

Back to Afzelia

Pi 0.015

MarkerSeek score 59.70

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 16
pi: 0.015
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	465-899 bp	1.0	84.1
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	468-902 bp	1.0	82.6
psbZ-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	461-895 bp	1.0	82.5
psbZ-trnG-UCC_p4	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	486-920 bp	1.0	82.3
psbZ-trnG-UCC_p5	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	466-900 bp	1.0	82.2