Hotspot

trnC-GCA-petN

LSC · 29281-30309 · 1029 bp

Back to Afzelia

Pi 0.00298

MarkerSeek score 63.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 7
pi: 0.00298
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1072-1102 bp	1.0	80.7
trnC-GCA-petN_p2	TTTATCGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	1125-1155 bp	1.0	79.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1123-1153 bp	1.0	78.8
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1090-1120 bp	1.0	78.5
trnC-GCA-petN_p5	TTTATCGTTTTGGCGGCATG	TCTTCCCCATACTACGAGGG	45.0	55.0	57.7	57.6	1176-1206 bp	1.0	77.2