Hotspot

atpH-atpI

LSC · 14336-15740 · 1405 bp

Back to Afzelia

Pi 0.0185

MarkerSeek score 70.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1405
variable_sites: 45
pi: 0.0185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1308-1735 bp	1.0	85.1
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1274-1701 bp	1.0	85.1
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1357-1784 bp	1.0	84.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1323-1750 bp	1.0	84.0
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1275-1702 bp	1.0	83.9