Hotspot

trnK-UUU-rps16

LSC · 4328-5212 · 885 bp

Back to Afzelia

Pi 0.00561

MarkerSeek score 68.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 11
pi: 0.00561
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	58.6	58.0	878-1030 bp	1.0	80.1
trnK-UUU-rps16_p2	GAGTCGCACTTAAAAGCCGA	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	58.6	58.0	879-1031 bp	1.0	80.0
trnK-UUU-rps16_p3	TCGAGTCGCACTTAAAAGCC	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	58.6	58.0	881-1033 bp	1.0	80.0
trnK-UUU-rps16_p4	TCGCACTTAAAAGCCGAGTA	CAAAAAGGGCGGGTGTTTTT	45.0	45.0	57.3	58.0	876-1028 bp	1.0	78.8
trnK-UUU-rps16_p5	CGCACTTAAAAGCCGAGTAC	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	57.2	58.0	875-1027 bp	1.0	78.4