Hotspot

trnH-GUG-psbA

LSC · 87-384 · 298 bp

Back to Afzelia

Pi 0.088

MarkerSeek score 79.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 40
pi: 0.088
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCCAT	55.0	55.0	58.1	58.0	238-398 bp	1.0	90.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	252-412 bp	1.0	90.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	251-411 bp	1.0	90.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	253-413 bp	1.0	90.9
trnH-GUG-psbA_p5	GCTATGTTATGGGCGAACGA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	58.1	58.0	302-462 bp	1.0	90.8