Hotspot

ndhE

SSC · 124054-124359 · 306 bp

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Pi 0.00327

MarkerSeek score 28.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GTAGACACCAGACGAAGCAG	GATTTACCAGGGCCAACACA	55.0	50.0	58.0	58.1	1627-1669 bp	1.0	81.0
ndhE_p2	GTAGACACCAGACGAAGCAG	AGCATTTCTGGGGTCAGTTC	55.0	50.0	58.0	57.8	1595-1637 bp	1.0	80.1
ndhE_p3	GTAGACACCAGACGAAGCAG	GCATTTCTGGGGTCAGTTCT	55.0	50.0	58.0	57.8	1594-1636 bp	1.0	80.1
ndhE_p4	GAGTCGGGTGTGTGAATGAA	GATTTACCAGGGCCAACACA	50.0	50.0	57.8	58.1	1504-1546 bp	1.0	80.0
ndhE_p5	CCAGACGAAGCAGTGGTTTA	GATTTACCAGGGCCAACACA	50.0	50.0	57.8	58.1	1620-1662 bp	1.0	79.8