Hotspot

rps11-rpl36

LSC · 83908-84029 · 122 bp

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Pi 0.0219

MarkerSeek score 60.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 4
pi: 0.0219
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GAACCAATACGCCCATTCCT	GCGCGGACGAATTATAGTGA	50.0	50.0	57.9	58.2	231-234 bp	1.0	79.7
rps11-rpl36_p2	AATACGCCCATTCCTACGTG	GCGCGGACGAATTATAGTGA	50.0	50.0	57.8	58.2	226-229 bp	1.0	78.3
rps11-rpl36_p3	CAATACGCCCATTCCTACGT	GCGCGGACGAATTATAGTGA	50.0	50.0	57.8	58.2	227-230 bp	1.0	78.3
rps11-rpl36_p4	TGAACCAATACGCCCATTCC	GCGCGGACGAATTATAGTGA	50.0	50.0	58.2	58.2	232-235 bp	1.0	78.2
rps11-rpl36_p5	TTACGTGAACCAATACGCCC	GCGCGGACGAATTATAGTGA	50.0	50.0	58.3	58.2	237-240 bp	1.0	77.9