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clpP1-psbB

LSC · 76287-76745 · 459 bp

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Pi 0.00327

MarkerSeek score 58.60

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 3
pi: 0.00327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	ACCCAAGTTGCATCTTCCTC	GCAACTAGGGCTGTATGCAT	50.0	50.0	57.8	58.0	612-613 bp	1.0	78.8
clpP1-psbB_p2	ATCTTCCTCTCCGGGATTCC	GCAACTAGGGCTGTATGCAT	55.0	50.0	58.3	58.0	601-602 bp	1.0	78.2
clpP1-psbB_p3	AAGTTGCATCTTCCTCTCCG	GCAACTAGGGCTGTATGCAT	50.0	50.0	57.6	58.0	608-609 bp	1.0	77.3
clpP1-psbB_p4	ACCCAAGTTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	540-541 bp	1.0	77.2
clpP1-psbB_p5	ATCTTCCTCTCCGGGATTCC	CGATACCAAGGCAAACCCAT	55.0	50.0	58.3	58.2	529-530 bp	1.0	76.6