Hotspot

trnG-GCC-trnfM-CAU

LSC · 39398-39670 · 273 bp

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Pi 0.0159

MarkerSeek score 53.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 273
variable_sites: 8
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CGGATTGTGAGGCCCATTAA	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	510-541 bp	1.0	84.8
trnG-GCC-trnfM-CAU_p2	TGTGTGGACATATGCATGCA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.9	58.0	386-417 bp	1.0	84.4
trnG-GCC-trnfM-CAU_p3	TGTGTGGACATATGCATGCA	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.9	58.1	380-411 bp	1.0	83.9
trnG-GCC-trnfM-CAU_p4	CGGATTGTGAGGCCCATTAA	CTTCGTCCATAAGTTCCCGG	50.0	55.0	57.9	58.1	578-609 bp	0.75	74.3
trnG-GCC-trnfM-CAU_p5	TGTGTGGACATATGCATGCA	CTTCGTCCATAAGTTCCCGG	45.0	55.0	57.9	58.1	454-485 bp	0.75	74.0