Hotspot

atpF-atpH

LSC · 13996-14511 · 516 bp

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Pi 0.0158

MarkerSeek score 51.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 16
pi: 0.0158
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGTGGCCCAAGTAAACGAAA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.6	58.0	600 bp	1.0	79.3
atpF-atpH_p2	CAGTGGCCCAAGTAAACGAA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.4	58.0	601 bp	1.0	78.9
atpF-atpH_p3	TGGCCCAAGTAAACGAAAGA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.4	58.0	598 bp	1.0	77.0
atpF-atpH_p4	GTGGCCCAAGTAAACGAAAG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.3	58.0	599 bp	1.0	76.5
atpF-atpH_p5	TTAAACCCGAAACTTCCGGC	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.8	58.0	628 bp	1.0	75.9