Hotspot

rps15-ycf1

SSC · 123036-123448 · 413 bp

Back to Aethionema

Pi 0.0417

MarkerSeek score 72.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 30
pi: 0.0417
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	GCGTTTTCAATTGCGATGGA	50.0	45.0	58.0	58.0	1218-1306 bp	1.0	88.7
rps15-ycf1_p2	GATCCCCTGCTTTCGTCTTT	GCGTTTTCAATTGCGATGGA	50.0	45.0	57.9	58.0	1088-1176 bp	1.0	88.2
rps15-ycf1_p3	TCTGTGCACCAATATCTGCC	GCGTTTTCAATTGCGATGGA	50.0	45.0	58.0	58.0	1789-1869 bp	1.0	87.5
rps15-ycf1_p4	TAAGCGAAGAACACCATGCA	GCGTTTTCAATTGCGATGGA	45.0	45.0	57.8	58.0	1488-1568 bp	1.0	87.1
rps15-ycf1_p5	CCCATCGCGTTACATAAGGT	GCGTTTTCAATTGCGATGGA	50.0	45.0	57.8	58.0	1615-1695 bp	1.0	86.5