Hotspot

ndhG-ndhI

SSC · 118366-118722 · 357 bp

Back to Aethionema

Pi 0.0383

MarkerSeek score 69.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 357
variable_sites: 25
pi: 0.0383
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	GCGGCAAGGTATATTGGTCA	50.0	50.0	58.0	58.0	979-994 bp	0.75	72.8
ndhG-ndhI_p2	CCCCCACGTAAATAAGGAGC	GCGGCAAGGTATATTGGTCA	55.0	50.0	58.0	58.0	1000-1015 bp	0.75	72.6
ndhG-ndhI_p3	GCCCCCACGTAAATAAGGAG	GCGGCAAGGTATATTGGTCA	55.0	50.0	58.0	58.0	1001-1016 bp	0.75	72.6
ndhG-ndhI_p4	GTTATGGCCCCCACGTAAAT	GCGGCAAGGTATATTGGTCA	50.0	50.0	57.9	58.0	1007-1022 bp	0.75	72.4
ndhG-ndhI_p5	ACGAAGTAATCCCATCCCCA	GCGGCAAGGTATATTGGTCA	50.0	50.0	58.1	58.0	1099-1114 bp	0.75	71.8