Hotspot

rpl32-trnL

SSC · 113171-113694 · 524 bp

Back to Aethionema

Pi 0.0478

MarkerSeek score 84.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 524
variable_sites: 43
pi: 0.0478
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCGTTGAAAGCGTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	57.2	57.9	625-641 bp	1.0	80.8
rpl32-trnL_p2	TCGTTGAAAGCGTTTTCGTT	AGCAGCGTGTCTACCAATTT	40.0	45.0	57.2	57.8	618-634 bp	1.0	80.4
rpl32-trnL_p3	TCGTTGAAAGCGTTTTCGTT	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	57.2	57.8	624-640 bp	1.0	80.2
rpl32-trnL_p4	TCGTTGAAAGCGTTTTCGTT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	57.2	57.8	623-639 bp	1.0	80.2
rpl32-trnL_p5	TCGTTGAAAGCGTTTTCGTT	GAGCAGCGTGTCTACCAATT	40.0	50.0	57.2	58.3	619-635 bp	1.0	79.6