Hotspot

ndhF-rpl32

SSC · 112150-113011 · 862 bp

Back to Aethionema

Pi 0.0343

MarkerSeek score 74.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 862
variable_sites: 54
pi: 0.0343
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGT	CGAATACGCTTTTTCGAGATCG	45.0	45.5	55.2	58.2	933-996 bp	1.0	47.4
ndhF-rpl32_p2	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGA	45.0	40.0	55.2	55.5	934-997 bp	1.0	47.4
ndhF-rpl32_p3	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGATC	45.0	40.9	55.2	57.2	934-997 bp	1.0	47.4
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGAT	45.0	38.1	55.2	56.1	934-997 bp	1.0	47.4
ndhF-rpl32_p5	TGGAAGTGGAATGAAAGGTATGA	CGAATACGCTTTTTCGAGATCG	39.1	45.5	57.5	58.2	932-995 bp	1.0	47.4