Hotspot

rpl36-rps8

LSC · 78845-79334 · 490 bp

Back to Aethionema

Pi 0.0299

MarkerSeek score 70.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 27
pi: 0.0299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACGGAAGCCCTT	GTCGGCGGAGAAATTTTGTG	45.0	50.0	57.7	58.0	544-553 bp	1.0	82.9
rpl36-rps8_p2	TTTTACGAACGGAAGCCCTT	AGTCGGCGGAGAAATTTTGT	45.0	45.0	57.7	58.1	545-554 bp	1.0	82.3
rpl36-rps8_p3	TAATTCGTCCCCTCCTACGG	GTCGGCGGAGAAATTTTGTG	55.0	50.0	58.3	58.0	586-595 bp	1.0	82.1
rpl36-rps8_p4	TAATTCGTCCCCTCCTACGG	AGTCGGCGGAGAAATTTTGT	55.0	45.0	58.3	58.1	587-596 bp	1.0	81.6
rpl36-rps8_p5	GTCCCCTCCTACGGATTAGT	GTCGGCGGAGAAATTTTGTG	55.0	50.0	57.6	58.0	580-589 bp	1.0	81.6