Hotspot

trnK-trnQ

LSC · 4308-6419 · 2112 bp

Back to Aethionema

Pi 0.0326

MarkerSeek score 73.70

Primer available yes

Label trnK-trnQ

Feature Metadata

label_name: trnK-trnQ
feature_type: igs
region: LSC
length_bp: 2112
variable_sites: 127
pi: 0.0326
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-trnQ_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2170-2277 bp	1.0	85.4
trnK-trnQ_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2169-2276 bp	1.0	85.4
trnK-trnQ_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	2156-2263 bp	1.0	83.1
trnK-trnQ_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	2181-2288 bp	1.0	82.1
trnK-trnQ_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	2179-2286 bp	1.0	80.9