Hotspot

trnH-psbA

LSC · 83-346 · 264 bp

Back to Aethionema

Pi 0.0721

MarkerSeek score 73.30

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 264
variable_sites: 28
pi: 0.0721
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	GTTGATAGTCAAGGGCGTGT	55.0	50.0	58.1	57.9	376-439 bp	1.0	88.3
trnH-psbA_p2	GATCCACTTGGCTACATCCG	GGTGTAGCTGGTGTATTCGG	55.0	55.0	58.1	58.1	694-757 bp	1.0	86.5
trnH-psbA_p3	CAATCCACTGCCTTGATCCA	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	708-761 bp	0.75	74.8
trnH-psbA_p4	AATCCACTGCCTTGATCCAC	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	707-760 bp	0.75	74.8
trnH-psbA_p5	ACAATCCACTGCCTTGATCC	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	709-762 bp	0.75	74.8