Hotspot

ndhF

SSC · 110889-113111 · 2223 bp

Back to Aesculus

Pi 0.00424

MarkerSeek score 45.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 32
pi: 0.00424
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AGAACCGGTGAATCAGAAACA	50.0	42.9	58.0	57.5	2536-2754 bp	0.9	69.3
ndhF_p2	AGGGGGACTAAACAGGAACA	AGAACCGGTGAATCAGAAACA	50.0	42.9	57.9	57.5	2426-2644 bp	0.9	68.6
ndhF_p3	CGAGGAAACGGAACAGATCC	AGAACCGGTGAATCAGAAACA	55.0	42.9	58.4	57.5	2337-2555 bp	0.9	66.8
ndhF_p4	ACGGAAGAAAGGGAGGAAGA	AGAACCGGTGAATCAGAAACA	50.0	42.9	58.0	57.5	2480-2698 bp	0.8	65.4
ndhF_p5	GGAGACATCCACATGCGAAA	AGAACCGGTGAATCAGAAACA	50.0	42.9	58.3	57.5	2353-2913 bp	0.7	59.5