Hotspot

rpl32-trnL-UAG

SSC · 114325-115364 · 1040 bp

Back to Aesculus

Pi 0.0111
MarkerSeek score 60.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1040
variable_sites
36
pi
0.0111
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGGTCAAAGCTTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 994-1193 bp 1.0 85.4
rpl32-trnL-UAG_p2 GCGGTCAAAGCTTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 995-1194 bp 1.0 85.4
rpl32-trnL-UAG_p3 AGCATCCCGAAAACAGACAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1501-1692 bp 1.0 85.1
rpl32-trnL-UAG_p4 AGCATCCCGAAAACAGACAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1502-1693 bp 1.0 85.1
rpl32-trnL-UAG_p5 GCATCCCGAAAACAGACAGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1500-1691 bp 1.0 85.0