Hotspot

accD-psaI

LSC · 59853-60542 · 690 bp

Back to Aesculus

Pi 0.0132

MarkerSeek score 62.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 27
pi: 0.0132
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTATTTCAGCTCCACGCCTT	GGCACAAAAAGGGAGGGTAA	50.0	50.0	58.0	58.0	757-788 bp	1.0	85.0
accD-psaI_p2	CTATTTCAGCTCCACGCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	792-823 bp	1.0	84.6
accD-psaI_p3	CTATTTCAGCTCCACGCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	798-829 bp	1.0	83.9
accD-psaI_p4	CTATTTCAGCTCCACGCCTT	CACGAAAGGCACAAAAAGGG	50.0	50.0	58.0	58.1	764-795 bp	1.0	83.8
accD-psaI_p5	CTATTTCAGCTCCACGCCTT	CCACGAAAGGCACAAAAAGG	50.0	50.0	58.0	58.1	765-796 bp	1.0	83.8