Hotspot

psbZ-trnG-GCC

LSC · 36492-36994 · 503 bp

Back to Aesculus

Pi 0.0514

MarkerSeek score 78.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 53
pi: 0.0514
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	618-694 bp	1.0	88.9
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	621-697 bp	1.0	87.3
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	614-690 bp	1.0	87.2
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	619-695 bp	1.0	86.9
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	617-693 bp	1.0	86.1