Hotspot

trnM-CAU-psbD

LSC · 31788-33205 · 1418 bp

Back to Aesculus

Pi 0.00801

MarkerSeek score 59.70

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 1418
variable_sites: 34
pi: 0.00801
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.1	57.8	1544-1562 bp	1.0	80.1
trnM-CAU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.1	57.8	1547-1565 bp	1.0	79.9
trnM-CAU-psbD_p3	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1575-1593 bp	1.0	79.7
trnM-CAU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1578-1596 bp	1.0	79.6
trnM-CAU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1577-1595 bp	1.0	79.4