Hotspot

atpF-atpH

LSC · 12895-13258 · 364 bp

Back to Aesculus

Pi 0.00952

MarkerSeek score 56.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 12
pi: 0.00952
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCGAACCAAGGAAACGAAAG	CGAGGCGGAGGGAAAAATAC	50.0	55.0	58.0	58.7	513-521 bp	1.0	78.7
atpF-atpH_p2	GCGAACCAAGGAAACGAAAG	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	512-520 bp	1.0	78.7
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAC	50.0	55.0	57.9	58.7	543-551 bp	1.0	77.8
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	542-550 bp	1.0	77.8
atpF-atpH_p5	AGCGAACCAAGGAAACGAAA	GAGGCGGAGGGAAAAATACG	45.0	55.0	58.3	58.7	513-521 bp	1.0	76.4