Hotspot

trnS-GCU-trnG-UCC

LSC · 7923-8707 · 785 bp

Back to Aesculus

Pi 0.0106
MarkerSeek score 53.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
785
variable_sites
26
pi
0.0106
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCGACGCTTTAATCCACTCA TGAATGAAACTGAAGGACCCC 50.0 47.6 58.0 57.8 892-905 bp 1.0 74.1
trnS-GCU-trnG-UCC_p2 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 847-860 bp 1.0 73.6
trnS-GCU-trnG-UCC_p3 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 847-860 bp 1.0 73.6
trnS-GCU-trnG-UCC_p4 GCTCGTACAACGGATTAGCA TGAATGAAACTGAAGGACCCC 50.0 47.6 58.1 57.8 914-927 bp 1.0 73.4
trnS-GCU-trnG-UCC_p5 GCTCGTACAACGGATTAGCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 869-882 bp 1.0 72.9