Hotspot

ycf4

LSC · 60652-61221 · 570 bp

Back to Aeschynomene

Pi 0.169

MarkerSeek score 82.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 570
variable_sites: 93
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	TTCGAAACCAAAGAAGGCCA	45.0	45.0	58.0	57.9	1121 bp	0.5	59.3
ycf4_p2	ATTTCCGGCAATTGCAATGG	CGCGGGAGTTAGTCTTTGAA	45.0	50.0	58.0	57.9	1308 bp	0.5	59.2
ycf4_p3	ATTTCCGGCAATTGCAATGG	TTCGCGGGAGTTAGTCTTTG	45.0	50.0	58.0	57.9	1310 bp	0.5	59.2
ycf4_p4	ATTTCCGGCAATTGCAATGG	CGTGTTTCCGGGTATTCCTT	45.0	50.0	58.0	57.9	1713 bp	0.5	59.0
ycf4_p5	CGGACAGTAGGCACAAATCA	TTCGAAACCAAAGAAGGCCA	50.0	45.0	57.9	57.9	1057 bp	0.5	58.8