Hotspot

ndhG-ndhI

SSC · 119651-120321 · 671 bp

Back to Aeschynomene

Pi 0.233

MarkerSeek score 79.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 671
variable_sites: 125
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CACCTCCAACGGTCAAAAGA	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.0	58.1	1414 bp	0.5	59.3
ndhG-ndhI_p2	CAGCAGCTACAAACTGGGAA	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.1	58.1	1303 bp	0.5	58.8
ndhG-ndhI_p3	GCTACGACAATGGCACCTAT	TGGTCAACAAACAGTCCGAG	50.0	50.0	57.8	58.1	1643 bp	0.5	57.7
ndhG-ndhI_p4	CACCTCCAACGGTCAAAAGA	TTGCGGAAACTGTGTTGAGT	50.0	45.0	58.0	58.3	1129 bp	0.5	57.7
ndhG-ndhI_p5	GTTGTGCAGCAGCTACAAAC	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.2	58.1	1309 bp	0.5	57.7