Hotspot

rpl32-trnL-UAG

SSC · 114087-115011 · 925 bp

Back to Aeschynomene

Pi 0.258

MarkerSeek score 83.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 925
variable_sites: 227
pi: 0.258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGAGGATATTGGACAGCAT	CCGAAACAATCCAGCGAGTA	50.0	50.0	57.8	58.0	1371-1923 bp	1.0	88.7
rpl32-trnL-UAG_p2	AAGCGAGGATATTGGACAGC	CCGAAACAATCCAGCGAGTA	50.0	50.0	57.8	58.0	1373-1925 bp	1.0	88.1
rpl32-trnL-UAG_p3	GCGAGGATATTGGACAGCAT	TGGTAAATGGCCCGAAACAA	50.0	45.0	57.8	58.0	1382 bp	0.5	58.6
rpl32-trnL-UAG_p4	AAGCGAGGATATTGGACAGC	TGGTAAATGGCCCGAAACAA	50.0	45.0	57.8	58.0	1384 bp	0.5	58.0
rpl32-trnL-UAG_p5	GCGAGGATATTGGACAGCAT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.8	58.1	1525 bp	0.5	57.9