Hotspot

psaJ-rpl33

LSC · 67756-68242 · 487 bp

Back to Aeschynomene

Pi 0.133

MarkerSeek score 82.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 62
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCATTGATATTCCCCTT	CGGATATCTTTACCCTTGGCC	40.0	52.4	53.0	58.2	543-560 bp	1.0	51.2
psaJ-rpl33_p2	GATGCATTGATATTCCCCTTT	CGGATATCTTTACCCTTGGCC	38.1	52.4	53.9	58.2	543-560 bp	1.0	51.2
psaJ-rpl33_p3	GATGCATTGATATTCCCCTTTT	CGGATATCTTTACCCTTGGCC	36.4	52.4	54.8	58.2	543-560 bp	1.0	51.2
psaJ-rpl33_p4	TGCATTGATATTCCCCTTTT	CGGATATCTTTACCCTTGGCC	35.0	52.4	52.7	58.2	541-558 bp	1.0	51.2
psaJ-rpl33_p5	TGCATTGATATTCCCCTTTTT	CGGATATCTTTACCCTTGGCC	33.3	52.4	53.7	58.2	541-558 bp	1.0	51.2