Hotspot

ycf4-cemA

LSC · 61222-61713 · 492 bp

Back to Aeschynomene

Pi 0.147

MarkerSeek score 78.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 61
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGGATGTGGGGAGTGGTTAT	TAGGAATTCTTGAACCCGCG	50.0	50.0	58.0	58.0	1220 bp	0.5	59.7
ycf4-cemA_p2	GCCGCTTTCTTGGATGTAGA	TAGGAATTCTTGAACCCGCG	50.0	50.0	58.0	58.0	957 bp	0.5	59.7
ycf4-cemA_p3	GGAAGTCTTAGGACAGGGGT	TAGGAATTCTTGAACCCGCG	55.0	50.0	58.0	58.0	1045 bp	0.5	59.5
ycf4-cemA_p4	TGGAAGTCTTAGGACAGGGG	TAGGAATTCTTGAACCCGCG	55.0	50.0	58.0	58.0	1046 bp	0.5	59.5
ycf4-cemA_p5	TGGATGTGGGGAGTGGTTAT	CTTGAACCCGCGAGTTAAGA	50.0	50.0	58.0	57.9	1212 bp	0.5	59.2