Hotspot

trnQ-UUG-rps16

LSC · 55837-56377 · 541 bp

Back to Aeschynomene

Pi 0.196

MarkerSeek score 82.70

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 99
pi: 0.196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAATCAAAAATGGGGCGTGG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.0	58.0	679-683 bp	1.0	91.9
trnQ-UUG-rps16_p2	AGCAGCTTGCCAAACAAAAG	GTCTTTCAAGTCGCACGTTG	45.0	50.0	58.1	58.0	1252-1305 bp	1.0	91.5
trnQ-UUG-rps16_p3	TAGGCTTCCGGTAATTTCGC	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	1170-1223 bp	1.0	91.5
trnQ-UUG-rps16_p4	GAATCAAAAATGGGGCGTGG	AAACTGGCAGCAACATACCA	50.0	45.0	58.0	58.0	1086-1087 bp	1.0	91.3
trnQ-UUG-rps16_p5	GAATCAAAAATGGGGCGTGG	AACTCCTCGTATTCGCAACC	50.0	50.0	58.0	58.0	1346 bp	0.5	59.9