Hotspot

psbD-trnT-GGU

LSC · 28695-29980 · 1286 bp

Back to Aeschynomene

Pi 0.0983

MarkerSeek score 89.50

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 119
pi: 0.0983
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1437-1480 bp	1.0	88.7
psbD-trnT-GGU_p2	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1438-1481 bp	1.0	88.7
psbD-trnT-GGU_p3	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1439-1482 bp	1.0	88.6
psbD-trnT-GGU_p4	CACAAAAACGAAACGGTCCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1440-1483 bp	1.0	88.5
psbD-trnT-GGU_p5	CCACAAAAACGAAACGGTCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1441-1484 bp	1.0	88.5