Hotspot

trnG-GCC-psbZ

LSC · 24860-25378 · 519 bp

Back to Aeschynomene

Pi 0.134
MarkerSeek score 83.90
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
519
variable_sites
66
pi
0.134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA ATTCTTAATTCTCTCATTTCTTGA 50.0 25.0 57.8 52.2 590-871 bp 1.0 50.1
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA GGTATTCTTAATTCTCTCATTTCTTGA 50.0 29.6 57.8 55.8 593-874 bp 1.0 50.0
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA GGTATTCTTAATTCTCTCATTTCTTG 50.0 30.8 57.8 54.5 593-874 bp 1.0 50.0
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA GGTATTCTTAATTCTCTCATTTCT 50.0 29.2 57.8 52.3 593-874 bp 1.0 50.0
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA GGTATTCTTAATTCTCTCATTTCTT 50.0 28.0 57.8 53.1 593-874 bp 1.0 50.0