Hotspot

ycf3-psaA

LSC · 18712-19585 · 874 bp

Back to Aeschynomene

Pi 0.121

MarkerSeek score 84.00

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 100
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCAGGTAATT	ATGCCACTCAGCCAAAGAAA	50.0	45.0	58.0	58.0	1204 bp	0.5	60.0
ycf3-psaA_p2	CTATTCCTATTCCGACCGCG	ATGCCACTCAGCCAAAGAAA	55.0	45.0	58.0	58.0	1306 bp	0.5	59.8
ycf3-psaA_p3	AGCTATAGCCCTTACCCCAG	ATGCCACTCAGCCAAAGAAA	55.0	45.0	58.0	58.0	1210 bp	0.5	59.7
ycf3-psaA_p4	AGCCCTTACCCCAGGTAATT	AACCCTGCCAAATGGTGATT	50.0	45.0	58.0	58.0	1546 bp	0.5	59.7
ycf3-psaA_p5	AGCCCTTACCCCAGGTAATT	GGCACTAGGTCCAATGTGAG	50.0	55.0	58.0	58.0	1277 bp	0.5	59.7