Hotspot

trnL-UAA-trnT-UGU

LSC · 13694-15062 · 1369 bp

Back to Aeschynomene

Pi 0.201

MarkerSeek score 81.00

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1369
variable_sites: 270
pi: 0.201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	CGGAGCGTGATTGGTTTCTA	50.0	50.0	58.1	58.0	1893-2698 bp	1.0	90.9
trnL-UAA-trnT-UGU_p2	TGTCGTCAACCCCATTTGTT	CGGAGCGTGATTGGTTTCTA	45.0	50.0	57.9	58.0	2094 bp	0.5	59.5
trnL-UAA-trnT-UGU_p3	GTCTACGCGGATCCATTTGT	CGGAGCGTGATTGGTTTCTA	50.0	50.0	58.1	58.0	2567 bp	0.5	59.5
trnL-UAA-trnT-UGU_p4	CTACGCGGATCCATTTGTGA	CGGAGCGTGATTGGTTTCTA	50.0	50.0	58.1	58.0	2565 bp	0.5	59.4
trnL-UAA-trnT-UGU_p5	TCTACGCGGATCCATTTGTG	CGGAGCGTGATTGGTTTCTA	50.0	50.0	58.1	58.0	2566 bp	0.5	59.4