Hotspot

rpl16

LSC · 82745-84352 · 1608 bp

Back to Aerides

Pi 0.00357

MarkerSeek score 63.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1608
variable_sites: 11
pi: 0.00357
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTCCAGAGATAAAGAAATCCGT	ACGAACAATTAGAATTCTATAAGGT	39.1	28.0	57.3	54.4	1743-1786 bp	1.0	42.4
rpl16_p2	TGTCCAGAGATAAAGAAATCCGT	AACGAACAATTAGAATTCTATAAGGT	39.1	26.9	57.3	55.1	1744-1787 bp	1.0	42.4
rpl16_p3	TGTCCAGAGATAAAGAAATCCGT	ACGAACAATTAGAATTCTATAAGGTT	39.1	26.9	57.3	55.1	1743-1786 bp	1.0	42.4
rpl16_p4	TCTGTCCAGAGATAAAGAAATCCG	ACGAACAATTAGAATTCTATAAGGT	41.7	28.0	58.1	54.4	1745-1788 bp	1.0	42.4
rpl16_p5	CTGTCCAGAGATAAAGAAATCCG	ACGAACAATTAGAATTCTATAAGGT	43.5	28.0	56.8	54.4	1744-1787 bp	1.0	42.4