Pi
0.000969
MarkerSeek score
59.60
Primer available
yes
Label
ndhB
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhB_p1 | CATAACTCCAGCCCATTCCC | CCTATTCATGGGGATTCCGT | 55.0 | 50.0 | 58.0 | 56.7 | 2237-2260 bp | 1.0 | 70.8 |
| ndhB_p2 | CATAACTCCAGCCCATTCCC | TCCTATTCATGGGGATTCCGT | 55.0 | 47.6 | 58.0 | 58.3 | 2238-2261 bp | 1.0 | 70.5 |
| ndhB_p3 | CTCCAGCCCATTCCCATTTT | CCTATTCATGGGGATTCCGT | 50.0 | 50.0 | 58.1 | 56.7 | 2232-2255 bp | 1.0 | 69.9 |
| ndhB_p4 | CTCCAGCCCATTCCCATTTT | TCCTATTCATGGGGATTCCGT | 50.0 | 47.6 | 58.1 | 58.3 | 2233-2256 bp | 1.0 | 69.5 |
| ndhB_p5 | CATAACTCCAGCCCATTCCC | TCCTATTCATGGGGATTCCG | 55.0 | 50.0 | 58.0 | 56.5 | 2238-2261 bp | 1.0 | 68.5 |
| ndhB_p1 | CCTATTCATGGGGATTCCGT | CATAACTCCAGCCCATTCCC | 50.0 | 55.0 | 56.7 | 58.0 | 2237-2260 bp | 1.0 | 70.8 |
| ndhB_p2 | TCCTATTCATGGGGATTCCGT | CATAACTCCAGCCCATTCCC | 47.6 | 55.0 | 58.3 | 58.0 | 2238-2261 bp | 1.0 | 70.5 |
| ndhB_p3 | CCTATTCATGGGGATTCCGT | CTCCAGCCCATTCCCATTTT | 50.0 | 50.0 | 56.7 | 58.1 | 2232-2255 bp | 1.0 | 69.9 |
| ndhB_p4 | TCCTATTCATGGGGATTCCGT | CTCCAGCCCATTCCCATTTT | 47.6 | 50.0 | 58.3 | 58.1 | 2233-2256 bp | 1.0 | 69.5 |
| ndhB_p5 | TCCTATTCATGGGGATTCCG | CATAACTCCAGCCCATTCCC | 50.0 | 55.0 | 56.5 | 58.0 | 2238-2261 bp | 1.0 | 68.5 |