Hotspot

petD-rpoA

LSC · 79192-79376 · 185 bp

Back to Aerides

Pi 0.0306

MarkerSeek score 74.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 9
pi: 0.0306
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTTTTGATCGGTACCGCAGT	TTGAGCACTGTACAGAAGCA	45.0	45.0	57.8	57.1	335-338 bp	1.0	74.2
petD-rpoA_p2	GTCCAGTAGCCACAACAGTC	TTGAGCACTGTACAGAAGCA	55.0	45.0	58.2	57.1	355-358 bp	1.0	73.8
petD-rpoA_p3	TCCAGTAGCCACAACAGTCT	TTGAGCACTGTACAGAAGCA	50.0	45.0	58.3	57.1	354-357 bp	1.0	73.2
petD-rpoA_p4	GGTACCGCAGTAGCTCTTTG	TTGAGCACTGTACAGAAGCA	55.0	45.0	58.4	57.1	326-329 bp	1.0	72.8
petD-rpoA_p5	GTACCGCAGTAGCTCTTTGG	TTGAGCACTGTACAGAAGCA	55.0	45.0	58.4	57.1	325-328 bp	1.0	72.8