Hotspot

clpP-psbB

LSC · 72774-73614 · 841 bp

Back to Aerides

Pi 0.0429

MarkerSeek score 92.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 63
pi: 0.0429
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 92.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	995-1070 bp	1.0	88.7
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	941-1016 bp	1.0	87.6
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	917-992 bp	1.0	87.4
clpP-psbB_p4	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	981-1056 bp	1.0	87.1
clpP-psbB_p5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	966-1041 bp	1.0	86.8