Hotspot

petA-psbJ

LSC · 63423-64361 · 939 bp

Back to Aerides

Pi 0.0087

MarkerSeek score 68.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 16
pi: 0.0087
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	1018-1052 bp	1.0	78.0
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	1019-1053 bp	1.0	77.9
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	1043-1077 bp	1.0	76.4
petA-psbJ_p4	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	1044-1078 bp	1.0	76.4
petA-psbJ_p5	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	1041-1075 bp	1.0	75.7