Hotspot

accD-psaI

LSC · 58909-59799 · 891 bp

Back to Aerides

Pi 0.0101

MarkerSeek score 51.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 17
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.0	58.0	1187-1205 bp	1.0	82.4
accD-psaI_p2	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	1250-1268 bp	1.0	82.3
accD-psaI_p3	AATGCCTACATTGCGTTTGC	TCAACTAGCAGCATTCCGAC	45.0	50.0	58.0	58.0	1522-1540 bp	1.0	82.0
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TTGCTGAGACGGAAATACGG	45.0	50.0	58.0	58.0	1785-1803 bp	1.0	81.8
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TTGCTGAGACGGAAATACGG	55.0	50.0	58.0	58.0	1848-1866 bp	1.0	81.7