Hotspot

matK-trnK-UUU

LSC · 3273-4397 · 1125 bp

Back to Aerides

Pi 0.00582

MarkerSeek score 65.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 13
pi: 0.00582
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	50.0	50.0	57.0	57.9	1267-1278 bp	1.0	73.0
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.0	58.2	1265-1276 bp	1.0	72.5
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	50.0	50.0	57.0	57.6	1269-1280 bp	1.0	70.7
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	50.0	50.0	57.0	57.6	1271-1282 bp	1.0	70.7
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	47.6	50.0	57.5	57.9	1267-1278 bp	1.0	69.2